Masuho Lab

The GPCR Signaling System in Health and Disease

  • Research
  • Projects
  • Publications
  • Protocols
  • Links

 Molecular Biology

    • The Genetic Codon
    • Dpn I Digestion of the Template DNA
    • PCR with PfuUltra DNA Polymerase
    • Mutagenesis
    • Colony PCR 
    • Plasmid Purification with NucleoBond Xtra Midi Plus EF
    • Shipping of plasmids on filter paper and their recovery

    Reagents

    • Recipes for reagents

    Cell Biology

    • Culture of 293T/17 cells 
    • Quick Transfection Procedure for 293T/17 cells
    • Coating Procedure of Coverslips

    Templates

    •  Plasmid File

    Biochemistry

    • Stripping Immunoblots for Reprobing
    • Storing Working Antibodies

    Softwares

    • ImageJ
    • Preparing high resolution images for web from PowerPoint slides

    Powered by Squarespace